Thermotolerance and Genetic Stability of Extremophilic Microorganisms Isolated from Geothermal Environments
Keywords:
Thermotolerance, extremophiles, geothermal environments, genetic stability, thermophilic microorganisms, heat shock proteins, genomic adaptationAbstract
Extremophilic microorganisms, particularly thermophiles and hyperthermophiles, demonstrate remarkable resilience under high-temperature environments, making them key models for understanding molecular adaptations to thermal stress. This study investigates the thermotolerance and genetic stability of extremophilic strains isolated from geothermal sites, focusing on both physiological resilience and genomic integrity under sustained thermal exposure. By employing phenotypic assays, whole-genome sequencing, and comparative genomics, we characterize the growth kinetics, mutation rates, and adaptive mechanisms of selected isolates. Results indicate the presence of unique chaperonins, DNA repair systems, and heat-shock regulatory networks that collectively ensure survival and genome stability at elevated temperatures. These findings hold significant implications for biotechnological applications in industrial processes requiring robust microbial performance under thermophilic conditions.
References
(1) Brock, T.D., Freeze, H. (1969). Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile. Journal of Bacteriology, 98(1), 289–297.
(2) Stetter, K.O. (1999). Extremophiles and their adaptation to hot environments. FEBS Letters, 452(1-2), 22–25.
(3) Canganella, F., Wiegel, J. (2011). Extremophiles: From abyssal to terrestrial ecosystems and possibly beyond. Naturwissenschaften, 98(4), 253–279.
(4) Cavicchioli, R. (2006). Extremophiles and the search for extraterrestrial life. Astrobiology, 6(3), 281–292.
(5) Atomi, H. (2005). Recent progress in the development of genetically manipulable thermophilic archaea. Applied Microbiology and Biotechnology, 64(6), 651–664.
(6) López-García, P., Moreira, D. (2006). Selective forces for the origin of the eukaryotic nucleus. Bioessays, 28(5), 525–533.
(7) Rothschild, L.J., Mancinelli, R.L. (2001). Life in extreme environments. Nature, 409(6823), 1092–1101.
(8) Daniel, R.M., Cowan, D.A. (2000). Biomolecular stability and life at high temperatures. Cellular and Molecular Life Sciences, 57(2), 250–264.
(9) Tindall, B.J. (2001). Thermophilic and hyperthermophilic microorganisms. Bergey’s Manual of Systematic Bacteriology, 2(1), 219–236.
(10) Huber, R., Stetter, K.O. (2001). Hyperthermophiles and their possible potential in biotechnology. Journal of Biotechnology, 83(1-2), 39–50.
(11) Forterre, P. (2002). A hot topic: the origin of hyperthermophiles. Cell, 109(4), 537–540.
(12) Schönheit, P., Buckel, W., Martin, W.F. (2016). On the origin of heterotrophy. Trends in Microbiology, 24(1), 12–25.
(13) Lecompte, O., et al. (2002). Comparative analysis of ribosomal proteins in complete genomes: an example of reductive evolution at the domain scale. Nucleic Acids Research, 30(24), 5382–5390.
(14) Makarova, K.S., et al. (2001). Comparative genomics of the Archaea (Euryarchaeota): evolution of conserved protein families, the stable core, and the variable shell. Genome Research, 11(7), 1019–1034.
(15) Holden, J.F., Baross, J.A. (1995). Enhanced thermotolerance and temperature-induced proteins in the hyperthermophile Archaeoglobus fulgidus. Applied and Environmental Microbiology, 61(11), 4046–4051.
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